Determination of carbohydrate-deficient transferrin in human serum with capillary zone electrophoresis
نویسندگان
چکیده
منابع مشابه
Determination of carbohydrate-deficient transferrin using capillary zone electrophoresis.
BACKGROUND Current methods for carbohydrate-deficient transferrin (CDT) often suffer from low precision, complexity, or risk of false positives attributable to genetic variants. In this study, a new capillary zone electrophoresis (CZE) method for CDT was developed. METHODS CZE was performed on a P/ACE 5000 using fused-silica capillaries [50 microm (i.d.) x 47 cm] and the CEOFIX CDT buffer sys...
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To assist the diagnosis of high risk alcohol consumption or alcohol dependence, particularly in the absence of evidences of recent alcohol intake (relapse), objective biochemical markers are today available, which, if used correctly, may reduce the degree of subjectivity that the adoption of merely clinical and psychological diagnostic criteria shows inevitably. The present paper reviews briefl...
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The performance of high-resolution CZE for determination of carbohydrate-deficient transferrin (CDT) in human serum based on internal and external quality data gathered over a 10-year period is reported. The assay comprises mixing of serum with a Fe(III) ion-containing solution prior to analysis of the iron saturated mixture in a dynamically double-coated capillary using a commercial buffer at ...
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Capillary zone electrophoresis (CZE) with a dynamic double coating formed by charged polymeric reagents represents an effective tool for the separation of iron-saturated transferrin (Tf) isoforms and thus the determination of carbohydrate-deficient transferrin (CDT, sum of asialo-, monosialo- and disialo-Tf in relation to total Tf) in human serum. Using the CEofix-CDT reagents, a 50 microm inne...
متن کاملDetermination of carbohydrate-deficient transferrin levels by using capillary electrophoresis in a Korean population.
BACKGROUND Carbohydrate-deficient transferrin (CDT) levels have rarely been determined in an Asian population. We evaluated the analytical performance of a test for measuring CDT levels by using capillary electrophoresis (EP). METHODS We determined the precision of CDT measurement by using capillary EP and nephelometry and compared the CDT values obtained using both the methods. We included h...
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ژورنال
عنوان ژورنال: Journal of Chromatography A
سال: 2008
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2008.05.025